Detection of Small Targets in Sea Clutter Based on RepVGG and Continuous Wavelet Transform

Constructing a high-performance target detector under the background of sea clutter is always necessary and important. In this work, we propose a RepVGGA0-CWT detector, where RepVGG is a residual network that gains a high detection accuracy. Different from traditional residual networks, RepVGG keeps an acceptable calculation speed. Giving consideration to both accuracy and speed, the RepVGGA0 is selected among all the variants of RepVGG. Also, continuous wavelet transform (CWT) is employed to extract the radar echoes' time-frequency feature effectively. In the tests, other networks (ResNet50, ResNet18 and AlexNet) and feature extraction methods (short-time Fourier transform (STFT), CWT) are combined to build detectors for comparison. The result of different datasets shows that the RepVGGA0-CWT detector performs better than those detectors in terms of low controllable false alarm rate, high training speed, high inference speed and low memory usage. This RepVGGA0-CWT detector is hardware-friendly and can be applied in real-time scenes for its high inference speed in detection

Evapotranspiration and its components over a rainfed spring maize cropland under plastic film on the Loess Plateau, China

Aim of study: To determine seasonal variations in evapotranspiration (ET) and its components; and ascertain the key factors controlling ET and its components in a rainfed spring maize field under plastic film.Area of study: Shouyang County in Shanxi Province on the eastern Loess Plateau, ChinaMaterial and methods: Eddy covariance system combined with micro-lysimeters and meteorological observing instruments were used in the field. The manual method was used to measure the green leaf area index (GLAI) during the growing season.Main results: In 2015 and 2016, the growing season ET accounted for 80% and 79% of annual ET, respectively. Soil evaporation (E) accounted for 36% and 33% of the growing season ET in 2015 and 2016, respectively. The daily crop coefficient increased with increasing GLAI until a threshold of ~3 m2 m−2 in the canopy-increasing stage, and decreased linearly with decreasing GLAI in the canopy-decreasing stage. At equivalent GLAI, daily basal crop coefficient and soil water evaporation coefficient were generally higher in the canopy-increasing and -decreasing stages, respectively. During the growing season, the most important factor controlling daily ET, T, and E was net radiation (Rn), followed by GLAI for daily ET and T, and soil water content at 10-cm depth for daily E; during the non-growing season, daily ET was mainly controlled by Rn.Research highlights: The daily crop coefficient and its components reacted differently to GLAI in the canopy-increasing and -decreasing stages

An Evolutionary Analysis of B-Box Transcription Factors in Strawberry Reveals the Role of FaBBx28c1 in the Regulation of Flowering Time

Flowering connects vegetative and generative developmental phases and plays a significant role in strawberry production. The mechanisms that regulate strawberry flowering time are unclear. B-box transcription factors (BBXs) play important roles in the flowering time regulation of plants. Nevertheless, BBXs in octoploid cultivated strawberry (Fragaria ananassa) and their functions in flowering time regulation have not been identified. Here, we identified 51 FaBBXs from cultivated strawberry and 16 FvBBXs from diploid wild strawberry (Fragaria vesca), which were classified into five groups according to phylogenetic analysis. Further evolutionary analysis showed that whole-genome duplication or segmental duplication is a crucial factor that leads to the expansion of the BBX gene family in two strawberry species. Moreover, some loss and acquisition events of FaBBX genes were identified in the genome of cultivated strawberry that could have affected traits of agronomic interest, such as fruit quality. The promoters of FaBBX genes showed an enrichment in light-responsive, cis-regulatory elements, with 16 of these genes showing changes in their transcriptional activity in response to blue light treatment. On the other hand, FaBBX28c1, whose transcriptional activity is reduced in response to blue light, showed a delay in flowering time in Arabidopsis transgenic lines, suggesting its role in the regulation of flowering time in cultivated strawberry. Our results provide new evolutionary insight into the BBX gene family in cultivated strawberry and clues regarding their function in flowering time regulation in plants

An Evolutionary Analysis of B-Box Transcription Factors in Strawberry Reveals the Role of FaBBx28c1 in the Regulation of Flowering Time

Flowering connects vegetative and generative developmental phases and plays a significant role in strawberry production. The mechanisms that regulate strawberry flowering time are unclear. B-box transcription factors (BBXs) play important roles in the flowering time regulation of plants. Nevertheless, BBXs in octoploid cultivated strawberry (Fragaria ananassa) and their functions in flowering time regulation have not been identified. Here, we identified 51 FaBBXs from cultivated strawberry and 16 FvBBXs from diploid wild strawberry (Fragaria vesca), which were classified into five groups according to phylogenetic analysis. Further evolutionary analysis showed that whole-genome duplication or segmental duplication is a crucial factor that leads to the expansion of the BBX gene family in two strawberry species. Moreover, some loss and acquisition events of FaBBX genes were identified in the genome of cultivated strawberry that could have affected traits of agronomic interest, such as fruit quality. The promoters of FaBBX genes showed an enrichment in light-responsive, cis-regulatory elements, with 16 of these genes showing changes in their transcriptional activity in response to blue light treatment. On the other hand, FaBBX28c1, whose transcriptional activity is reduced in response to blue light, showed a delay in flowering time in Arabidopsis transgenic lines, suggesting its role in the regulation of flowering time in cultivated strawberry. Our results provide new evolutionary insight into the BBX gene family in cultivated strawberry and clues regarding their function in flowering time regulation in plants

Identification of the GRAS gene family in the Brassica juncea genome provides insight into its role in stem swelling in stem mustard

GRAS transcription factors are known to play important roles in plant signal transduction and development. A comprehensive study was conducted to explore the GRAS family in the Brassica juncea genome. A total of 88 GRAS genes were identified which were categorized into nine groups according to the phylogenetic analysis. Gene structure analysis showed a high group-specificity, which corroborated the gene grouping results. The chromosome distribution and sequence analysis suggested that gene duplication events are vital for the expansion of GRAS genes in the B. juncea genome. The changes in evolution rates and amino acid properties among groups might be responsible for their functional divergence. Interaction networks and cis-regulatory elements were analyzed including DELLA and eight interaction proteins (including four GID1, two SLY1, and two PIF3 proteins) that are primarily involved in light and hormone signaling. To understand their regulatory role in growth and development, the expression profiles of BjuGRASs and interaction genes were examined based on transcriptome data and qRT-PCR, and selected genes (BjuGRAS3, 5, 7, 8, 10, BjuB006276, BjuB037910, and BjuA021658) had distinct temporal expression patterns during stem swelling, indicating that they possessed diverse regulatory functions during the developmental process. These results contribute to our understanding on the GRAS gene family and provide the basis for further investigations on the evolution and functional characterization of GRAS genes

Hexokinase1: A glucose sensor involved in drought stress response and sugar metabolism depending on its kinase activity in strawberry

Hexokinase1 (HXK1) is a bifunctional enzyme that plays indispensable roles in plant growth, nitrogen utilization, and stress resistance. However, information on the HXK family members of strawberries and their functions in glucose sensing and metabolic regulation is scarce. In the present study, four HXKs were firstly identified in the genome of Fragaria vesca and F. pentaphylla. The conserved domains of the HXK1s were confirmed, and a site-directed mutation (S177A) was introduced into the FpHXK1. FpHXK1, which shares the highest identity with the AtHXK1 was able to restore the glucose sensitivity and developmental defects of the Arabidopsis gin2-1 mutant, but not its kinase-activity-impaired mutant (FpHXK1S177A). The transcription of FpHXK1 was dramatically up-regulated under PEG-simulated drought stress conditions. The inhibition of the HXK kinase activity delayed the strawberry plant’s responses to drought stress. Transient overexpression of the FpHXK1 and its kinase-impaired mutant differentially affected the level of glucose, sucrose, anthocyanins, and total phenols in strawberry fruits. All these results indicated that the FpHXK1, acting as a glucose sensor, was involved in drought stress response and sugar metabolism depending on its kinase activity

An effective method for establishing a regeneration and genetic transformation system for Actinidia arguta

The all-red A. arguta (Actinidia arguta) is an anthocyanin-rich and excellent hardy fruit. Many studies have focused on the green-fleshed A. arguta, and fewer studies have been conducted on the all-red A. arguta. Here we reported a regeneration and Agrobacterium-mediated transformation protocol by using leaves of all-red A. arguta as explants. Aseptic seedling leaves of A. arguta were used as callus-inducing materials. MS medium supplemented with 0.3 mg·L-1 2,4-D and 1.0 mg·L-1 BA was the optimal medium for callus induction of leaves, and medium supplemented with 3 mg·L-1 tZ and 0.5 mg·L-1 IAA was optimal for adventitious shoot regeneration. The best proliferation medium for adventitious buds was MS + 1.0 mg·L-1 BA + 0.3 mg·L-1 NAA. The best rooting medium was 1/2MS + 0.7 mg·L-1 IBA with a 100% rooting rate. For the red flesh hardy kiwi variety ‘Purpurna Saduwa’ (A. arguta var. purpurea), leaves are receptors for Agrobacterium (EHA105)-mediated transformation. The orthogonal experiment was used for the optimization of each genetic transformation parameter and the genetic transformation of the leaves was 21% under optimal conditions. Our study provides technical parameters for applying genetic resources and molecular breeding of kiwifruit with red flesh

FaGAPC2/FaPKc2.2 and FaPEPCK reveal differential citric acid metabolism regulation in late development of strawberry fruit

Citric acid is the primary organic acid that affects the taste of strawberry fruit. Glycolysis supplies key substrates for the tricarboxylic acid cycle (TCA cycle). However, little is known about the regulatory mechanisms of glycolytic genes on citric acid metabolism in strawberry fruits. In this study, the citric acid content of strawberry fruit displayed a trend of rising and decreasing from the initial red stage to the full red stage and then dark red stage. Thus, a difference in citric acid metabolic regulation was suspected during strawberry fruit development. In addition, overexpression of either cytoplasm glyceraldehyde-3-phosphate dehydrogenase (FxaC_14g13400, namely FaGAPC2) or pyruvate kinase (FxaC_15g00080, namely FaPKc2.2) inhibited strawberry fruit ripening and the accumulation of citric acid, leading to a range of maturity stages from partial red to full red stage. The combined transcriptome and metabolome analysis revealed that overexpression of FaGAPC2 and FaPKc2.2 significantly suppressed the expression of phosphoenolpyruvate carboxykinase (FxaC_1g21491, namely FaPEPCK) but enhanced the content of glutamine and aspartic acid. Meanwhile, the activities of PEPCK and glutamate decarboxylase (GAD) were inhibited, but the activities of glutamine synthase (GS) were increased in FaGAPC2/FaPKc2.2-overexpressed fruit. Further, functional verification demonstrated that overexpression of FaPEPCK can promote strawberry fruit ripening, resulting in a range of maturity stage from full red to dark red stage, while the citric acid synthase (CS) activities and citric acid content were significantly decreased. Overall, this study revealed that FaGAPC2/FaPKc2.2 and FaPEPCK perform an important role in reducing citric acid content in strawberry fruit, and FaGAPC2/FaPKc2.2 mainly by promoting the GS degradation pathway and FaPEPCK mainly by inhibiting the CS synthesis pathway

Apply the contour integral on multivalued function from the complex plane

This paper mainly intends to integrate the multivalued function on the complex plane by using contour integral. The improper integral of a single-valued function on the real plane is often difficult to solve directly. In addition to solving the integral itself, we also need to judge its convergence and divergence. In order to solve integral of this kind, this paper extends its domain onto the complex plane. Then, we can find that this kind of function may become a complex multivalued function on the complex plane. However, if we use the contour integral, we can deal with the complex-valued function efficiently and then obtain the solution of the integral. Using contour integral to solve the integral in the complex field is applied in many fields. Contour integral can be used to solve partial differential equations, which can be applied in electrodynamics, quantum mechanics, hydrodynamics, and other fields

Investigation and Ecological Evaluation of Plant Resources on Campus of Jiamusi University

The purpose of the systematic investigation of plant resources in Jiamusi University campus is to understand the composition, community characteristics and ecological distribution of plant resources in Jiamusi University, and to make rational use of them. In the process of investigation, the methods of actual step-by-step search and data search are adopted to carry out ecological evaluation. According to statistics, there are 113 kinds of plants on Jiamusi University campus, of which 93 are woody plants and 18 are herbaceous plants. Its growth environment is suitable for forest margin, undergrowth, hillside, and shrub and so on. Its main uses are greening, shading, dust prevention and noise reduction. It has high value. It is hoped that the research will provide a basis for the future construction of ecological civilization on campus